Jubei Yagyu is a reccuring character in the Samurai Shodown franchise, first introduced in original game.

A seasoned warrior, he is the one-eyed master of Yagyuu Shinkage-ryuu, and a close friend of Hanzo Hattori. He is a fictionalized interpretation of the real-life samurai and folk hero, Yagyuu Juubei Mitsuyoshi, the son of the originator of the real life kenjutsu martial arts style Yagyuu Shinkage-ryuu, Yagyuu Tajima no Kami Munenori, who personally taught the style both to the Tokugawa Shogunate, including the Tokugawa's personal shinobi squadron, the Oniwabanshu, and is often romanticized to have wandered throughout Japan as a free spirit like Miyamoto Musashi or deployed on secret missions by the Shogunate.

Jubei is an experienced, battle-hardened warrior who is loyal to the Tokugawa shogunate, even as a ronin. He enjoys seeing potential in younger warriors and encourages them to one day surpass him. Unlike his comrade in arms, Hanzo, Jubei is a more care-free man when off-duty, and he enjoys times of peace with a hearty laugh.